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Sample Paper on Organic

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Sample Paper on Organic

Purpose statement

 The purpose of this lab is to determine the number of components in a mixture, the identity of compounds and the purity of a compound. Also, observing the appearance of a product’s progress is monitored.

  1. Chemicals / Physical Data / Hazards

Chemical structure


Functional Groups Present


(Irritant, corrosive, toxic, etc.)

Acetylsalicylic acid


Carboxylic acid, ester, phenol




Ester, xanthine

Mildly toxic



Ester, amide




Amide phenol



  1. Procedure

Place a 8ml test mixture to be analyzed in a beaker, place a filter paper into the solution, then cover the beaker with an aluminous foil so that the so that saturation to course evaporation in the filter chamber. The TLC plate is emersed in a mixture of a solvent in a preparation chamber such that only a small bit is disposed to the liquid while the rest of the plate remains The liquid finds its way up the upper side of the TLC plate though an action chemists refer to as capillary action (Srivastava, 57).

When moving up past the spotted areas on the TLC plate, each component of the solvent  finds it focal point for each component that are later absorbed by the molecules of the components which are in solution form. (Nag,120). Generally, the components’ solubility varies such that the rate at which they are absorbed to the adsorbent is fast enough to the up plate than others. On reaching the top, the solvent causes the plate to be withdraws away from the developing chamber and later giving room for the component that were separated be undergo visualization. When found out to be colored then the resulting outcome of the exercise is said to be straightforward. On instances when the compounds are not colored further viewing is recommended through the use of other devices such as the UV lamps.

Interactions such as ion-dipole, dipole-dipole, hydrogen bonding, and dipole induced dipole, and van der Waals forces are dependent on the strength of organic compounds that bind them to the adsorbent. On the case of silica gel, the force that overpowers the others is the interactive forces such as the dipole-dipole type. The polar molecules that are weak move through the adsorbent quickly than the polar species (Schwarzenbach, Philip and Dieter, 460)

  • Srivastava, ManMohan. High-performance Thin-Layer Chromatography (hptlc). Springer: Berlin.2011. pp. 54-60

Nag, A. Analytical and Instrumental Techniques in Agriculture, Environmental and Food Engineering. S.l.: Prentice-Hall Of India, 2016.pp 120

Schwarzenbach, René P, Philip M. Gschwend, and Dieter M. Imboden. Environmental Organic Chemistry. Hoboken: John Wiley & Sons, 2005.pp 460


  1. 1. Filter paper saturates the solvent in the development chamber’s air with solvent vapor so the stationary phase doesn’t dry during the process.
  2. an aromatic hydrocarbon is predicted to move up the TLC plate faster compared to alcohol because of the high polarity and the fast components.
  3. Hexanes, ethyl acetate, and acetic acid
  4. Pens are not recommended because the ink will travel up the TLC plate with the TLC solvent, just like your chemical samples do. One can spot the mark TLC plates using a pencil because the graphite in a pencil will not run up the plate.
  5. it is because a compound will have different affinities for the mobile and stationary thus the temperature, depth of mobile phase, nature of the TLC plate are varied.
  6. This aides to distinguish cases in which two substances are found to have the identical Rf value so that one understands that they have different compounds.







Works cited

Srivastava, ManMohan. High-performance Thin-Layer Chromatography (hptlc). Springer: Berlin.2011. pp. 54-60

Nag, A. Analytical and Instrumental Techniques in Agriculture, Environmental and Food Engineering. S.l.: Prentice-Hall Of India, 2016.pp 120

Schwarzenbach, René P, Philip M. Gschwend, and Dieter M. Imboden. Environmental Organic Chemistry. Hoboken: John Wiley & Sons, 2005.pp 460


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