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Sample Assignment Paper on Wildlife Diseases (Fall 2014)

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Sample Assignment Paper on Wildlife Diseases (Fall 2014)
  1. (5) In the lifecycle of Histomonasmeleagridis, I said that there are definitive, intermediate, and paratenic hosts. Defend or refute the selections I presented in lecture.           Histomonas meleagrids is a flagellate parasite that commonly attacks poultry more especially turkeys, peafowls and pheasant and its main organ of attack being the caecum, liver and sometimes the kidneys and the spleen. After they have been passed out in the feces, these organisms do not live for long. However, these parasites are able to be enclosed in the eggshells of fecal poultry, after which they can get new hosts. They are transported within the egg of H.gallinarum.The which carries the transmission of H.gallinarum.After this, multiplication occurs then development within the H.gallinarum.The H.meleagrids is taken  in the body and multiplies in the intestines and finally it invades the germinative area of the nematodes ovary, where it  feeds and then goes to the oocysts and penetrates them. After that it is then ejected from body system of the worm. The proper definitive host then feeds on the worm’s eggs. These hosts which include: chicken, turkey, peafowl and pheasant which then hatch in the intestines. The earthworms serve as the paratenic host for Histomonas meleagrids.The earthworms will feed on the nematode eggs which later hatch releasing second stage juveniles that become dormant in the body of the earthworm. In the intermediate host they attack heterak which is a caecal nematode.
  2. (5). What is the mode of transmission of Epizootic Hemorrhagic Disease in whitetail deer? In what states is EHD endemic and in what state have we seen epizootics? Predict (explain why) if EHD will continue to result in epizootics in Pennsylvania in the near and distant future.                                                                                                       Culicoides variipennis is the most common vector in North America in the Epizootic hemorrhage Disease. A deer must be bitten for it to be infected; it must be bitten by a midge carrying the virus. The disease is not directly transmitted from one deer to another, but must go through the insect vector. It has been observed that there exists single epizootic which do not recur. EHD is mainly transmitted through a culicoides, fly bite or midge. Epizootic hemorrhagic disease up to now has been found in a big number of deer in the United States. These deer are from Ottawa, Oceania, Iona, Berrien, Muskegon and Allegan. Some deer have also been reported to have died, and this has been associated with the disease. The estimation is I about 250 deer. EH is an acute infectious disease which affects some ruminant animals especially on the northern part of the United States of America and southern Canada. The  first time the disease was  diagnosed  was in 1955, after a big number of white tailed deer died in New Jersey and Michigan.                                                                                                                                     For long, it has been regarded as the new disease of the deer and the name epizootic was given in order to describe its pathological and clinical features. The disease has been in the United States for the last 50 years. In 1974,2006,2008,20009,2010,2011 and 2012, there occurred a number of deaths which were directly connected to EHD.In 2006, deaths occurred in the southwestern portion of the state of Allegan county, where an estimated of 50-75 deer died. In the year 2008, many deer died in the southeastern portion of Oakland and Macomb counties where an average of 150-200 deer died. In the whole of 2009, 300-450 deer died in the southwestern and south-central portion of the state in Livingston County. Other areas affected are St Joseph, Cass and other 30 counties on the southern half of the Lower Peninsula with about 14,898 deer dead. If the trend continues, the way the disease is spreading it will result in epizootics in Pennsylvania.
  3. 3. (10) Ebola virus is in the United States. Knowing what you know about this wildlife disease that can infect humans, answer the following questions:Is there a chance that it could become established in North American wildlife? What are precautions we should take to avoid spreading in humans? Once the potential epidemic is over, develop a monitoring program so that this virus can be monitored in the wild.                                                                                                                                                     The likelihood that Ebola disease would be established in North American wildlife is very rare because there is no interaction between the animals in the Congo where the disease is prevalent and the ones in the United States of America. The following  precautions need to be taken if you travel to an area that has infections of Ebola: Wash your hands frequently using alcohol based sanitizer in order to eliminate any traces of  the virus; avoid completely any contact with blood and other body  fluids of anyone especially the one who is sick whether diagnosed of the disease or not; avoid handling items that in any case may have come into contact with a person who is infected; avoid completely touching the body of somebody who has been confirmed that he died from Ebola; avoid touching bats and other primates that are not human ,including, their blood and fluids, and never touch even eat meat from these animals; avoid hospitals in west Africa, especially where the patients who are suffering from  Ebola are being treated; if you develop fever that temperatures of 101.5 degrees  celcius, and if you experience the following symptoms seek the services of a doctor immediately:headache,muscle pain,diarrhea,vomiting,stomach pain, or bruising or bleeding which cannot be explained; limit your contact with other people.

The best way to monitor the disease before it reaches humans, is to establish Ebola virus geographical distribution, identify the immunologically naïve populations. The interaction between the non human primates and humans should also be minimized because this contact is the one that brings about outbreaks in one part of the world and then human beings infect one another after they get the virus from the animals.                                                                                              4. (10). Compare the lifecycle and pathology in the appropriate wildlife of two of the following: Sphaeridiotremaglobulus, Ribeiroiaondatrae, Neascus spp., Clinostomum spp., and Dicrocoelium.

              These parasites produce black pigmentation that appears like black pepper and they are spread on the fins of fresh water lake and rivers in the United States of America and Africa. All the species of fish in Minnesota lakes and rivers are, in danger of being infected with the disease. The species of fish found in shallow waters are also affected. Only extra intestinal parasites are harmful to fish. In their life cycle, the birds that eat fish, release the eggs into the water. The eggs develop in the snails into intermediate stages; these parasites then swim and penetrate the muscles of fish and encyts.A black pigment is deposited on these fish. These infected fish are then eaten by birds and the life cycle begins once again. The adult worms are seen in fish eating birds. Clinostomum Spp, have a very complex life cycle, the adults live in the gut, the eggs come out with feces hatches, the miricidium penetrates the snail and then produces two sporocyts generations which then penetrate fish and form metacercarie throughout viscera H, where the infected fish is eaten by birds and then the cycle begins again. Both Species use birds as their main mode of transmission.

  1. (5). What is the reservoir of Parelaphostrongylus tenuisin Pennsylvania? What is a reservoir and explain why you chose that species (or group of species) as the reservoir. This refers to a brain worm in adult form which is also called a roundworm and it is usually found in the brain of different venous sinuses and subdural space of the brain in the white-tailed deer which are found in the Eastern North America. The Other animals affected are: moose wapiti (ilk), reindeer, mule, sheep, goats and guinea pig. However, these animals are abnormal hosts because; the worm frequently causes cerebrospinal nematodiasis, which is a disease of the nervous system hence resulting in death. I chose  the two because they are they are the most prevalent attackers of a number of animals in Eastern North America including white tailed deer, sheep and goats and even guinea pigs. They  therefore need closer attention I rdr to curb the deaths occurring.
  2. (5). Describe the lifecycle of Toxoplasma gondii and list 4 preventative measures that all pregnant women should follow to avoid contracting this wildlife and domestic disease.                                                                                                                                                   This is a protozoan disease which attacks a species of animals which are warm blooded including human beings and it can cause a disease called toxoplasmosis. The only known definive host for toxoplasmosia gondii is a group of members of the felidae-domestix cats and their relatives. The oocysts which are unsporulated are set in the feces of the cat.Oocysts take 1-5 days to sporulate in the environment, and then they become effective. The intermediate hosts include birds and rodents. They become infected after taking in soil, water or plant material which is contaminated. The oocysts the transform themselves into tachyzoites shortly after they have been taken in. They stay in the muscle tissues. The cats are also infected when they feed on intermediate hosts, which have tissue cysts. Cats can also be directly infected when they take in sporulated ocysts. The animals, which are kept for domestic use can also get infected with the tissue cysts after they consume sporulated oocysts in the environment. Pregnant women should take the following preventive measures: avoid eating uncooked meat from the animals that are infected; avoid taking anything that is contaminated with cat feces or the contaminated environment; avoid blood transfusions and make sure they are checked in hospitals because there can occur transplacentally infections from the mother to the child.
  3. (5) Compare the cuticle of nematodes with the tegument of (platyhelmithes). Flatworms have a tegument which is living. The outer layer is living because as a parasite, which lives in the gut of a host, it needs to absorb the nutrients from the medium, which is just around it. Therefore, it has an outer layer that is metabolically active for the absorption of the nutrients. The (Nemathelminthes) are different because they have an outer layer which combines with the muscles to form a hydroskeleton.It has a complete digestive system which begins in the mouth and ends in the anus.Therefore, both of them have digestive systems but the one for flatworms is  not as developed as the one of roundworms. The outer layer of flatworms is softer than the one of roundworms and is used for metabolism but the one of roundworms is not.
  4. (5). Please describe the following as they pertain to a particular anthropozoonotic wildlife disease: Infection, Infestation, Prevalence, Incidence, and Intensity. You pick the wildlife disease.                                                                                                                                           The Rhipecephalus disease, which attacks a number of wildlife in Africa in Zambia was the center of research recently. A total of 12 species of animals were researched on to assess the level of infections, the prevalence rates, infestation, the incidences of attack and their intensity. The prevalence rate was high in the animals in the worm species with more infections in their organs which showed that, their semi aquatic nature pronged their exposure in comparison with other animals. The incidents of the Kafue being attacked were very minimal, which was attributed to shorter attachment periods, as they spend most of their time submerged in water. In all the experiments, the intensity of the attack by Kobus Leche Kafuensis was high in the worm species.
  5. (5). Please explain: Can Disease Regulate Wildlife? The diseases can regulate the population of wildlife. If diseases attack animals and many of them die, definitely the population of the affected animals will go down. Equally, if the diseases that attack wild animals are well controlled, the population of the wild animals will go higher. For instance, a diseases like Epizooti Hemorrhage is not well controlled, can drastically reduce the population white tailed deer and other animals attacked by it in the United States where it is so prevalent. On the same note, if the disease like Neascus is not controlled, the fish will die hence regulating the population. This is because; this is a disease that has been attacking fish in the United States of America. Likewise, if these diseases are well managed, the population of the respective animals will increase. There is correlation between animal disease management and the population of the wildlife.

         10.(5) Compare vertical and horizontal transmission and then speculate if Edwardsiellatarda is transmissible through both methods.                                                                        Vertical transmission refers to an infection which is caused by a bacteria, virus and in some cases parasites. The disease is transmitted directly from the mother to a fetus during pregnancy or at child birth. Nutrional deficiencies can also aggravate the situation. Horizontal transmission occurs, through an infectious agent like bacteria, fungi or viral. The infection occurs between members of the same species but not in a mother child relationship. Horizontal transmission is evolving virulent and due to that, it is very important in evolutionary medicine.Edwardsiellatarda, is a bacteria disease that attacks humans and fish. It was known thirty years ago but still, little is known about the bacteria E tarda.It is transmitted horizontally.

  1. (5) Thinking about Cimexpipistrelli and Oeciacusvicarius makes me itchy. What do they vector and if they are (or could be) vectors of an infectious agent, do you think it would pass through the salivarian or stercorarian method? Explain why.                                                                        Oeciacusvius which is also called the swallow bug is a haemotaphagoas ectoparasite that mainly uses the cliff swallow as its host. They are found almost solely on cliff swallows. The nest cup is very important to the host and parasite relationship at any given time and stage of the host, nestling, nymph, adults and even eggs. In the life history of ectoparasites, they have evolved in a very close connection with those of the cliff swallow This is not harmful to some other swallows like the barn. Throughout the life cycle of the swallow bug, the adult and nymph stage take blood from their hosts especially at night. (Cimexpipistrelli) or bat bugs are roost octoparasite of the bats, which feed only on blood. They are found where the bats roost and not on the bodies of bats. They suck the blood of the bats as they roost and this is commonly done at night. Their mode of passage is salivarian. This is because they bite their hosts as they suck blood and in the process, they infect their hosts through the saliva. Stercovarian, is where the infection takes place through the feces of the parasitic insects which are involved.
  2. (5) I found the following ticks on my dog. What are they and what could they vector?

          The  smaller tick is the V.Dermacenter Variabilis tick which is female.It causes a disease called Lyme.The magnified one is an American dog tick  called Scapularis,deer tick or black legged tick ,and it causes a disease called Lyme.This tick is very small almost the size of a fleck    of black peper.They are very difficult to see in the dog and even when engorged due to their small size.They blow up like a big greyish or greenish  blob.


         13.(5). Trombiculids are incredibly annoying. Speculate to their current ability to regulate the population of a wildlife species and then enhance the ability of trombiculids to provide regulatory effects. (Essentially what would it take for them to provide more morbidity and mortality to that population).                                                                                                             Trombiculids are the larva stage of a very common type of mite which belongs in the family of Trombiculidae. This very tiny critters can, bite formidably and cause great pain which then is followed with irritation which is very intense. They take hours before they drop off ones skin. To control their populations, all yards of organic debris needs to be cleared, which will include clipping of lawns, pine straws and any leafy litter. The lawns and shrubs need to be sprayed in order to control them. In order to prevent them from landing on your skin, you need to use spray repellants. These mites do not fly but they jump into the body of humans. And they don’t burrow the human skin but they stay there and it’s only at the larva stage that they bite. They adults feed on insects and their eggs.

  1. 14. (10) compare the pathology of Neosporacaninum and Sarcocystis in wild ungulates and Anatids. Is it safe for hunters to eat meat infected with these protists?                                      Neospora   Caninum is a protozoan parasite  which is associated with the abortions that occur in bovines. The only definitive host known is the carnivorous animals. This is based on a sample of fecal, serum and brain that were tested. A number of animals were tested to certain the ones that are affected by Neosporacaninum. These animals included: otters, Mink and foxes. Out of all the animals tested using the brain tissue, only three percent of mink, six percent of foxes and four percent of otters tested positive. The results showed that the local ecology of a species does influence its epidemiological role. The disease has no treatment or vaccine. While Sarcocystis is the common protozoan parasites that affect dogs in the countries where they are endemic. It causes gastrointestinal and systemic disease. The prevalence rate in some countries is between 5-15.The parasite has a two host life history; however, sexual reproduction only occurs in the definitive host. The dogs are infected when they eat infected meat from other hosts like birds and herbivorous animals. Taking in of bradyzoites in food, which is not cooked, makes the oocysts to be released in the intestines of dogs where they sporulate and penetrate the walls of the intestines through the lymph nodes to skeletal muscles. The two have the same pathogens (protozoa), however, the animals attacked are different. This is because Neosporacaninum  attack  bovines, but the Sarcocysts attack dogs though, both are parasites. Equally, in the two, the oocysts are released in the intestines.                                                                                                             15. (15) please assess the worth for publication, using the attached departmental rubric, of one of the papers chosen by your classmates (you cannot choose to assess a paper you led in class). After that is completed, use the reviewers form (modified from Northeastern Naturalist) to convey your conclusions about the manuscripts merit for publication and summarize your comments on the manuscript.


Lab Report Rubric – Biology Department

Student: ___________________________ Date: ________________ Rater: ________________________


 [9-10 points]

 [7-8 points]

 [0-6 points]




·   Appropriate section headers

·   Well-organized; content in correct sections

·   Most section headers present

·   Generally organized, some mixing of sections

·   No section headers

·   Poorly organized



·   Demonstrates comprehension of problem

·   Provides thorough and relevant background

·   Hypothesis statement in testable form

·   Comprehends aspects of problem

·   Provides some relevant background

·   Hypothesis statement incomplete or requires clarification

·   Little comprehension of problem

·   Little or irrelevant background information

·   Hypothesis missing or not in testable form


Materials & Methods

·   Clearly and fully explains procedures, citing sources as needed

·   Explains procedures acceptably; may be missing some details or somewhat unclear

·   Procedures not adequate to repeat experiment


Data Presentation

·   All necessary tables & figures present and organized

·   Accurate headers, titles, legends

·   Minor absence of data or not clearly presented

·   Minor formatting errors for headers, titles, legends


·   Critical data missing or unclear

·   Absence of headers, titles, legends






·   Clearly summarizes all data, emphasizing key points

·   Correctly cites figures, tables

·   Summarizes data but may be unclear or incomplete

·   Insufficient reference to figures & tables

·   Missing significant results, not clearly explained

·   Fails to reference figures and tables






·   Fully interprets results in light of hypothesis

·   Addresses any problems with experiment clearly and appropriately

·   Creative suggestions for future research

·   Interprets results but not does fully address hypothesis

·   Mentions problems with experiment but explanation is insufficient

·   Minimal ideas for future research

·   Results poorly interpreted

·   Does not address problem areas

·   No suggestions for future research





·   Essentially free of errors in spelling/grammar

·   Written in concise, clear, scientific language


·   Minor errors (1-2 per page)

·   Minor wordiness, jargon, or casual language


·   Frequent or major errors (2+/page)

·   Notable wordiness, jargon, casual language



Use of Literature & Lit Cited

·   Effectively uses key literature

·   All sources cited correctly in text

·   Fully documented in approved style

·   Sufficient number of approved sources

·   Cites relevant sources

·   Sources cited in text with minor errors

·   Minor formatting issues

·   Sufficient number of approved sources

·   No use of literature or irrelevant sources

·   Sources poorly cited or not cited in text

·   Missing information or missing citations

·   Uses too few or unapproved sources



TOTAL ________


·   Clearly summarizes key points of paper without information not present elsewhere

·   Length as specified

·   Unclear, incomplete in minor aspects or contains minor new information

·   Slightly too long

·   Misses multiple key elements of paper or contains excessive new information

·   Notably too long or too short


Experimental Design

·   Creative and well-designed experiment.

·   Proper controls & adequate replication.

·   Adequate to test hypothesis.

·   Minor problems with controls or replication.

·   Experiment does not test hypothesis.

·   Improper controls, lacks replication.



·   Well-chosen statistical tests or comparisons

·   Accurately carried out and well-interpreted

·   Acceptable choice of statistical tests

·   Adequately carried out and interpreted

·   Clearly wrong test or no statistical results

·   Errors of execution or interpretation


Lab Report Rubric – detailed description

           Title: Clearly and briefly tells what paper is about. Identifies independent and dependent variables, study organism(s), [and study site]. Generally ~8-12 words.

              Organization: Well-organized report with section headers (Introduction, Methods, Results, Discussion). Correct information in each section.

             Introduction: Introduces topic and argues for its significance. Gives only necessary background information. Clearly states purpose or question and hypothesis/predictions.

           Methods: [Brief description of study site.] Study organism(s) clearly indicated, including scientific names if appropriate. Fully but briefly explains procedures and materials/equipment used; cites literature or lab manual for procedures not fully explicated. Sufficient to repeat experiment (with references).

             Results: Verbally summarizes results depicted in tables/figures, citing these properly. Emphasizes major patterns or trends in the data. Demonstrates that you comprehend the outcome of the lab or experiment.

           Tables: Clearly and neatly summarize data (and statistical results). Not a compilation of raw data (unless this is requested by instructor). Column and row headers clearly indicate what the values mean, and include units. Table title properly headed (e.g., Table 1: …), fully descriptive of data, and located above the table. Table formatting as specified by instructor (e.g., only horizontal borders, etc).

               Figures: Well-chosen to clearly depict major patterns in data (or as assigned). Axes labeled, including units. Legend present and properly labeled, but only if needed. Legend, equations, or other text do not obscure data. Colors, shades, and data points contrast adequately. No extraneous and distracting visual clutter (e.g., wild colors & patterns, 3-d bars, grey background, unnecessary gridlines). Any required elements (e.g., error bars, regression equations) present and properly executed. Figure title properly headed (Figure 1: …) and located beneath the figure.

             Discussion: Interprets results in light of the hypothesis. Discusses results and their significance. Indicates any conditions or events (e.g., small sample size, experimental errors) that may put results in question. Indicates questions that remain unresolved and suggests avenues of future research.

             Mechanics: Essentially free of spelling and grammatical errors. Scientific names formatted correctly. Written in concise, clear, scientific language, but not jargon-laden. Methods, results, and references to own results in discussion in past tense; present tense used to refer to published results. Uses appropriate and consistent voice. (Ecology journals generally prefer first-person, active voice.) Numbers formatted correctly (leading zeros, significant figures).

              Use of literature & literature cited: Effectively uses key literature in introduction and discussion. In-text citations in an approved style. Fully documents all sources cited in the paper, in consistent, approved style (e.g., CSE). Generally includes only peer-reviewed literature, published books, and lab manual; other sources as approved by instructor. Number of sources as required by instructor. Lit cited does not include sources not cited in paper.


Optional sections or grading criteria

Abstract: Briefly summarizes purpose, methods, major results, and conclusions. Does not cite literature. Does not include information not present elsewhere in the paper. Length as specified by instructor (generally 50-150 words).

Experimental Design: Well-designed to test hypothesis. Includes proper controls and adequate replication.

Statistics: Well-chosen statistical tests, or as assigned; correctly interprets statistical tests. Test results used to support biologically meaningful statements. Example: “Heart rates of Daphnia were significantly higher after 10-minute exposure to a 1% caffeine solution (paired t-test, P = 0.0015).”


[Manuscript Number and Title]

Please return the manuscript copy, this summary form, and any additional sheet(s) of comments to me at the address on my cover letter. Your review/comments will be sent to the author(s) without your name attached.


Is the subject matter of this manuscript important and interesting enough and is its manner of presentation sufficient and rigorous enough to warrant publication?

YES                            Ticked



The manuscript is

                                    Acceptable after minor revision          Ticked______

                                    Acceptable after major revision          ______

                                    Unacceptable                          ______                                                         Summary comments:                                                                                                                                                                          The manuscript was well written from the beginning until the end. The heading given was very appropriate because it gives the reader a glimpse on what he is going to encounter in the text. The introduction is great with enough and detailed background information that creates a vivid picture on the focus the paper is going to have. In the body, the information is well elucidated with relevant examples, appropriate tables and graphs, which graphically represent the information which is being conveyed.                                                                                                                                                    The suggestions given seem realistic and practical if followed to the latter. Clear and appropriate scientific language is used which makes it a scientific paper. The sources cited, the explanations given and the procedures are appropriate for this manuscript. However, there are a few things that have to be looked at: The subject matter should delve deep into the issues and bring out the finer details so that the points can come out strongly; more sources should be given. Otherwise, this is a good manuscript that should be published.





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